High concordance of programmed death-ligand 1 expression with immunohistochemistry detection between antibody clones 22C3 and E1L3N in non-small cell lung cancer biopsy samples
Original Article

High concordance of programmed death-ligand 1 expression with immunohistochemistry detection between antibody clones 22C3 and E1L3N in non-small cell lung cancer biopsy samples

Wei Zhang#, Ziyang Cao#, Caixia Gao, Yan Huang, Chunyan Wu, Liping Zhang, Likun Hou

Department of Pathology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China

Contributions: (I) Conception and design: L Zhang, L Hou; (II) Administrative support: C Wu, Y H; (III) Provision of study materials or patients: C Gao, Z Cao; (IV) Collection and assembly of data: W Zhang, Z Cao; (V) Data analysis and interpretation: W Zhang, L Hou; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.

#These authors contributed equally to this work.

Correspondence to: Liping Zhang; Likun Hou. Department of Pathology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China. Email: Lipingzhang2002@outlook.com; 1600027@tongji.edu.cn.

Background: The detection of programmed death-ligand 1 (PD-L1) expression can enrich for patients who respond to anti-programmed cell death 1 (PD-1)/PD-L1 therapies. Though, for most laboratories, the cost of PD-L1 22C3 pharmDx is prohibitive for widespread use, whereas the laboratory-developed test (LDT) PD-L1 E1L3N antibody clone is widely available and inexpensive. This study aims to explore the analytical performance of E1L3N on the Dako Autostainer Link-48 platform and further evaluate the concordance of E1L3N and 22C3 expression in non-small cell lung cancer (NSCLC) biopsy samples.

Methods: A total of 171 NSCLC biopsy samples were utilized in this study. Cases with less than 100 tumor cells were excluded. Serial sections of representative blocks were used for immunohistochemistry (IHC) staining. The staining protocol was performed according to the standard PD-L1 IHC 22C3 pharmDx package. PD-L1 staining on the tumor cell membrane was detected by immunofluorescence.

Results: At a 1% cutoff value, PD-L1 was positive in 46.2% of patients using clone 22C3 and 42.1% of patients using E1L3N assays. At a 50% cutoff value, PD-L1 was positive in 16.4% of patients using clone 22C3 and 15.2% of the patients using E1L3N assays. Cohen’s kappa was used to evaluate the concordance of the PD-L1 expression between clone 22C3 and E1L3N. The kappa values were 0.893 [95% confidence interval (CI): 0.826–1] at the 1% cutoff and 0.868 (95% CI: 0.764–1) at the 50% cutoff. An evaluation of the intraclass correlation coefficients (ICCs) between the antibodies was used to quantify the interassay variability for PD-L1 expression in tumor cells. ICCs showed high concordance between the two antibodies (0.955, 95% CI: 0.939–0.967). Cohen’s kappa was also used to assess the consistency of the PD-L1 evaluation between two pathologists. The kappa values were 0.941 and 0.912 at the 1% cutoff, and 0.904 and 0.909 at the 50% cutoff for clone 22C3 and E1L3N expression, respectively.

Conclusions: The results indicated that the clone E1L3N assay has a high concordance with 22C3. The PD-L1 clone E1L3N assay is reliable and cost-effective, and could be used as a primary screening agent for PD-L1 IHC staining in pathological laboratories, especially in a research setting.

Keywords: PD-L1 (22C3); PD-L1 (E1L3N); immunohistochemistry (IHC); non-small cell lung cancer (NSCLC); biopsy

Submitted Jan 02, 2020. Accepted for publication Jul 27, 2020.

doi: 10.21037/tcr-20-101


Statistically, lung cancer has the highest incidence and mortality of all cancers among both sexes in the United States (1) and globally (2). Immunotherapy elucidates new treatment options for malignant tumors. Immune checkpoint inhibitors, such as anti-programmed cell death (PD-1) and anti-programmed death-ligand 1 (PD-L1), have proven to be highly effective in the treatment of different tumor types (3-5). The KEYNOTE-001 and KEYNOTE-024 trials demonstrated that the anti-PD-1 inhibitor, pembrolizumab, significantly prolonged progression-free survival and overall survival for previously untreated non-small cell lung cancer (NSCLC) patients, with at least 50% of the tumor cells expressing PD-L1. Remarkably, pembrolizumab monotherapy can be extended as a first-line therapy to locally-advanced or metastatic NSCLC patients, with 1% (or more) of the tumor cells expression PD-L1 (6). PD-L1 22C3 PharmDx, on the dedicated Dako Autostainer platform, is an approved companion diagnostic assay for the detection of the PD-L1 immunotherapy biomarker (7), in addition to epidermal growth factor receptor (EGFR), anaplastic lymphoma kinase (ALK) and ROS proto-oncogene 1 (ROS1), which need to be tested in advanced NSCLC patients.

PD-L1 protein expression is detected by immunohistochemistry (IHC). The PD-L1 Blueprint Projects 1 (8) and 2 (9) analyzed the concordance of PD-L1 clone expression across different detection platforms. Except for SP142, the PD-L1 clone 22C3, 28-8, and SP263 assays showed a similar expression pattern of tumor cells in NSCLC. Also, a real-world study that recruited 1,930 patients, including 412 lung cancer patients, reported that the 22C3 and 28-8 assays had analytical concordance (10). These studies indicate that 22C3, 28-8 and SP263 are interchangeable for the assessment of PD-L1 expression of tumor cells.

The E1L3N antibody clone has been detected in various malignant tumors including: melanoma (11), bladder (12), gastric (13), prostate (14), and breast (15). Previous studies have shown that E1L3N expression had a high concordance with the PD-L1 clones SP263 (16) and 28-8 (17). Several reports noted a close analytical performance between 22C3 and SP263 expression (18-21). However, different studies have reported conflicting results about evaluating the comparability of these PD-L1 clones assays. For example, clone 22C3 and SP263 assays (Dako and Ventana platform, respectively) exhibited a significant discrepancy in the positive percentage (22,23). Ma et al. reported that E1L3N showed poor staining of gastric tumor cells compared to SP142 and 28-8 in surgical specimens on different detection platforms (13). Similarly, the E1L3N assay using Ventana BenchMark XT automated platform showed lower PD-L1 positivity of bile duct tumor cells than SP263 and 22C3 assays in tissue microarrays (TMA) or whole tissue sections (24). The PD-L1 expression status in NSCLC biopsy samples using 22C3 and E1L3N antibodies on the Dako AutostainerLink-48 platform remains only partially understood. Moreover, standardized PD-L1 assays are costly, while PD-L1 clone E1L3N (for research use only) is inexpensive and widely available, and can be performed locally.

Therefore, this current study was undertaken to analyze and evaluate the analytical performance of the PD-L1 antibody clone E1L3N in comparison to the clone 22C3 on the Dako platform to assess its diagnostic value as a screening tool for NSCLC biopsy samples.

We present the following article in accordance with the STROBE reporting checklist (available at http://dx.doi.org/10.21037/tcr-20-101).


Patients and reagents

Our study was observational and retrospective and followed the World Medical Association’s Declaration of Helsinki (as revised in 2013) and approved by the Ethics Committee of Shanghai Pulmonary Hospital (No. k17-130, Shanghai, China). Informed consent was taken from all the patients.

One hundred seventy-one primary NSCLC patients were included in the present study and were enrolled in the Shanghai Pulmonary Hospital between May 2018 and September 2018. Clinical information data was collected from the electronic medical record management system, including age, gender, smoking history, stage, histological subtypes and targeted gene mutations. Cases with numbers of tumor cells (<100) were excluded. The PD-L1 antibody clone E1L3N was purchased from Cell Signaling Technology, and was derived from rabbit (Cat No. 13684S), and the immunogen was derived from intracellular peptides. The clone 22C3 was a Dako product (Cat No. M3653) that was derived from the extracellular peptides in mice (Cat No. S2022) (Figure S1).

Figure S1 Programmed death-ligand 1 (PD-L1) topology and the immunogen of both antibody clone E1L3N and 22C3.

Immunofluorescence staining

Sections were deparaffinized and rehydrated, and antigen retrieval was performed. The immunofluorescence staining was conducted with Dako mouse anti-PD-L1 22C3 (1:50) paired with Yeasen anti-mouse IgG labeled with Alexa Fluor 594 (1:100) and Cell Signaling Technology rabbit anti-PD-L1 E1L3N (1:400) paired with Yeasen anti-rabbit IgG labeled with Alexa Fluor 488 (1:100). All of the images were captured with ZEISS Imager.Z2 at a magnification of ×400.

Immunohistochemistry staining

Serial sections of biopsy samples were prepared for IHC. PD-L1 staining using the two primary antibodies was performed on the Dako AutostainerLink-48 platform, according to the manufacturer’s instructions. The concentrated antibody clone E1L3N was applied at a dilution of 1:400 using a visualization system (K8002, without LINKER). The concentrated antibody clone 22C3 was used at a dilution of 1:50 with a visualization system (K8002, with Mouse LINKER). The slides were counterstained with hematoxylin and mounted with cover slips.

Evaluation of PD-L1 tumor positive percentage

The assessment of PD-L1 expression was performed blindly by two senior thoracic pathologists who undertook a PD-L1 testing (22C3) and evaluation training course (22C3) of NSCLC samples. The PD-L1 staining results were evaluated using the tumor proportion score (TPS), with the final score for each assay being defined as the mean of the pathologists’ scores

The visualization of clear membranous positive staining in the tumor cells was classified as a positive result (excluding non-tumor cells and cytoplasmic staining of tumor cells) as described in the PD-L1IHC 22C3 pharmDx Interpretation Manual (25), irrespective of the staining intensities. Tonsil tissue was used as both a positive and negative control.

Statistical analysis

Scatterplots and Bland-Altman plots were used to show the concordance and differences between the two datasets. The plots were generated in Stata V14 and GraphPad Prism7. The overall percent agreement (OPA), positive percent agreement (PPA), negative percent agreement (NPA), and Cohen’s Kappa and Intra-Class Correlation (ICC) were calculated to determine the concordance of both datasets. All statistics were analyzed with SPSS v25.


Patient characteristics

A total of 171 primary NSCLC patients who underwent biopsies were analyzed, including 94 squamous cell carcinomas (SQC) and 65 adenocarcinomas (ADC). The median age of the participants was 63 years old (range, 23–88 years old), with 135 (78.9%, 135/171) males. The smoking rate was 38.6% (66/171). The proportion of early staged patients (stage I–II) was 3.5%. Targeted therapy-related driver genes were tested in 100 NSCLC cases (58.5%, 100/171), including EGFR, Kirsten rat sarcoma oncogene (KRAS), and B-RAF proto-oncogene (BRAF) mutation, and ROS1 and ALK fusion genes. The results are shown in Table 1.

Table 1
Table 1 Clinical-pathological characteristics of patients
Full table

TPS distribution of bothPD-L1 antibodies

TPS evaluation was performed according to the Agilent 22C3 pharmDx manual (25) and the technical companion assay (26,27). The three categories (0, 1–49%, ≥50%) are illustrated with representative images of the staining pattern (scanned by MoticEasyScan), as shown in Figure 1. Immunofluorescence staining of NSCLC biopsy samples (Figure 2) with clones 22C3 and E1L3N revealed specific positive staining for PD-L1 on the tumor cell membrane. E1L3N and 22C3 exhibited a strong correlation at the average PD-L1 expression (Figure 3A). The mean difference between the two assays in all cases was identified using Bland-Altman analysis (Figure 3B), and no significant differences were detected. E1L3N showed a high similarity with 22C3 at 1% and 50% cutoffs (Figure 3C).

Figure 1 Representative PD-L1 TPS for two clones at three cutoffs (immunohistochemistry, ×10). For 22C3, (A) PD-L1 with no staining (score: 0%); (B) PD-L1 score in the range of 1–49%; (C) PD-L1 score 50%. For E1L3N, (D) PD-L1 negative (score: 0%); (E) PD-L1 score in the range from 1–49%; (F) PD-L1 score 50%.
Figure 2 Immunofluorescence staining of PD-L1 antibodies on biopsy samples (40×). (A) Red signal for clone 22C3; (B) green signal for clone E1L3N.
Figure 3 Programmed death-ligand 1 (PD-L1) scoring distribution and comparison of the two clones expression. (A) PD-L1 TPS distribution (22C3 vs. E1L3N) by scatter plot; (B) the difference value (22C3 vs. E1L3N) by Bland-Altman plot; (C) Nonliar fit curve for two clones (22C3 vs. E1L3N).

Reproducibility of the pathologists’ evaluation scores

For the clone 22C3 and E1L3N assays, the two pathologists’ scores exhibited a high level of consistency (Figure 4A,B,C,D,E,F), with no significant differences detected (Pitman’s Test, P=0.120 and P=0.428, Figure 4). At the 1% and 50% cutoffs, the 22C3 expressions for Pathologist A were 47.4% and 46.8%, respectively, and 15.8% and 15.8% for Pathologist B, respectively. Also, for the clone E1L3N assay, it was evaluated with positivity rates of 41.5% and 43.9% for Pathologist A at the 1% and 50% cutoffs, respectively, and 15.8% and 14.6% for Pathologist B, respectively (Table 2). Both pathologists achieved high overall reliability in evaluating the two PD-L1 assays.

Figure 4 Programmed death-ligand 1 (PD-L1) scoring and the concordance between the two pathologists. (A) Scatter plot (Pathologist A vs. Pathologist B) for 22C3; (B) Bland-Altman plot (Pathologist A vs. Pathologist B) for 22C3; (C) Nonliar fit curve of the two pathologists (Pathologist A vs. Pathologist B) for 22C3; (D) Scatter plot of the two pathologists (Pathologist A vs. Pathologist B) for E1L3N; (E) Bland-Altman plot of the two pathologists (Pathologist A vs. Pathologist B) for E1L3N; (F) Nonliar fit curve of the two pathologists (Pathologist A vs. Pathologist B) for E1L3N.
Table 2
Table 2 Positive percentage of PD-L1 for two clones based on two cutoffs value
Full table

The concordance of both PD-L1 antibody clones and pathologists’ evaluations

The concordance of the clones’ expression and the pathologists’ evaluations were analyzed (Figure 5). All of the ICC values were greater than 0.9 (0.939, 0.977 and 0.977). At the 1% cutoff, Cohen’s kappa value for both antibody clones (22C3 and E1L3N) was 0.893. The percentages for the OPA, PPA, and NPA were 94.7%, 89.9%, and 98.9%, respectively. At the 50% cutoff, the Cohen’s kappa value was 0.868, and the percentages for the OPA, PPA and NPA were 96.5%, 85.7%, and 98.9%, respectively.

Figure 5 Forest plot for the concordance of the two clones and two pathologists.

For pathologists’s evaluation of clone 22C3 expression at the 1% and 50% cutoffs, the Cohen’s kappa values were 0.941 and 0.912, respectively, and for the clone E1L3N assay, the Cohen’s kappa values were 0.904 and 0.909 at the 1% and 50% cutoffs, respectively (Table 3).

Table 3
Table 3 The concordance of evaluating the two PD-L1 antibody clones between pathologists
Full table


Presently, the PD-L1 22C3 PharmDx assay, using the Dako Autostainer Link-48 platform, is approved by the Food and Drug Administration (FDA) as a diagnostic companion with pembrolizumab treatment. Though, since the cost of the PD-L1 22C3 PharmDx assay is prohibitive for most laboratories, more cost-effective alternatives are desirable. Considering that standardized assays are expensive and that not all platforms are available in most laboratories, pathologists strongly advocate for a comprehensive evaluation of the diagnostic performance of LDTs (28).

In this regard, harmonization studies that analyze the results obtained from different antibodies have been conducted. Since the clone 22C3 LDT achieved a high concordance with the 22C3 pharmDx assay on a dedicated Dako Autostainer Link-48 platform (18), the concordance of two LDTs expressions (between the clone 22C3 concentrated antibody and the clone E1L3N) is a comparable and meaningful way to analyze the results.

Several studies, such as the Blueprint Projects (8,9), have compared the PD-L1 antibody expression using different clones and testing platforms. Three other PD-L1 clones (28-8, SP263, and SP142) differ in terms of their IHC platform and detection systems. Different PD-L1 IHC assays have shown conflicting results (16,23). There may be several reasons for these discrepancies, including the expression heterogeneity in tumors, inter-clone differences, different platforms, and inter/intra-observer variability.

A recent study compared the LDT clone E1L3N to the 22C3 and SP263 assays to evaluate diagnostic accuracy. Compared to the 22C3 assay on a dedicated platform, the clone E1L3N showed a lower concordance at 1% and 50% cutoffs on the Ventana platform. However, E1L3N was highly consistent with the SP263 assays at both cutoffs using the Ventana platform (16). We found a high concordance of clone E1L3N and 22C3 expression at both the 1% and 50% cutoffs on the Dako Autostainer Link-48 platform. Also, clones E1L3N and 22C3 showed a high level of concordance by using quantitative immunofluorescence to assess PD-L1 expression. It is, therefore, reasonable to assume that the discrepancies between different PD-L1 IHC assays may be due to the different platforms and detection systems used.

The expression of clones E1L3N, SP142, and 28-8 was compared in surgically resected gastric cancer samples. The findings suggested that E1L3N expressed poor staining in both tumor and immune/stromal cells (13,29). Similarly, quantitative immunofluorescence was applied in resected NSCLC samples to assess the PD-L1 protein. E1L3N showed discordance with clone SP142 (30), which may be attributable to heterogeneity within tumors and antibody affinities. Using an immunohistochemical analysis of PD-L1 protein expression, the antibody E1L3N expressed a slightly higher statistically significant staining than that of 22C3 based on the average of the pathologists’ scores (31). The result demonstrates clone E1L3N is reproducible and that it is interchangeable with clone 22C3 for PD-L1 IHC staining.

Recent studies have evaluated PD-L1 expression between surgically resected samples and paired biopsies, and in these studies, PD-L1 clones exhibited a high level of concordance (32). Additionally, some TMA made from the surgically resected specimen also showed high consistency with the biopsy samples (33-35). These reports indicated that a biopsy is sufficient to represent the entire tumor for detecting the PD-L1 protein.

The high concordance between clone E1L3N and 22C3 expression was found in this study in three aspects. Firstly, the antibody’ design and products’ quality control objectively produced reliable assays from both antibody clones. Secondly, the two thoracic pathologists undertook a 22C3 IHC evaluation training course and had experience in cytology and biopsy diagnosis in clinical practice, these results meant that our data was reproducible, and high PPAs were achieved. Lastly, the limited tumor cell area made it easy for the pathologists to obtain an objective TPS compared to the resected NSCLC samples. A previous study found that the inter-pathologist variability was higher than assay variability (36). For this reason, further emphasis should be placed on the training of pathologists to improve their ability to evaluate PD-L1 protein expression (37).

In our study, we systemically compared the PD-L1 antibody E1L3N to the clone 22C3. We found a high concordance between E1L3N and 22C3 expression in NSCLC biopsy samples using IHC on the Dako Autostainer Link-48 platform, indicating that E1L3N could be applied for routine immunohistochemical analysis of PD-L1 detection in NSCLC. Currently, the clone E1L3N is labeled for research use only. Though, due to its cost-effectiveness and widespread availability, it is suitable for use in IHC and should be urgently standardized in the operating procedure for LDTs. The results observed in our study demonstrate that clone E1L3N is a promising and interchangeable alternative to the expensive and platform-dependent clone 22C3 assay, especially in research settings. Also, the intra-cellular domain of the E1L3N immunogen was different from 22C3, whose immunogen was located in the extra-cellular domain containing four glycosylated sites, which might affect the affinity between the PD-L1 epitope and its antibody.

Several limitations should be noted. This study had no outcome data based on of the actual PD-L1 expression status, and the sensitivity and specificity could not be calculated. Also, there was no validation by the commercial PD-L1 IHC 22C3 pharmDx, which is approved by the FDA. However, the concentrated PD-L1 antibody clone 22C3 achieved an almost equal potency with 22C3 pharmDx, and it was applied in most of the laboratories with companion instruments. Lastly, the lack of data available for the response to the anti-PD-1/anti-PD-L1 inhibitor therapy in these NSCLC patients presents a further limitation of this study.


In conclusion, the 22C3 and E1L3N PD-L1 antibody clones had a high concordance in the PD-L1 IHC staining in NSCLC biopsy samples, even though they were derived from different fragments of PD-L1 peptides and were sourced from different companies. Our results indicate that a more cost-effective LDT with E1L3N could be developed for the routine screening of PD-L1 expression status, especially in a research environment.


Funding: This study was partly supported by projects of the Science and Technology Commission of Shanghai Municipality (No. 18411962900), National Natural Science Foundation of China (No. 81702243 and No. 81802803) and Shanghai municipal health commission (No. 201740134 and No. 20184Y0222) and General program of Xinjiang Natural Science Foundation (2018D01C027).


Reporting Checklist: The authors have completed the STROBE reporting checklist. Available at http://dx.doi.org/10.21037/tcr-20-101

Data Sharing Statement: Available at http://dx.doi.org/10.21037/tcr-20-101

Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/tcr-20-101). The authors have no conflicts of interest to declare.

Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013), and approved by the Ethics Committee of Shanghai Pulmonary Hospital (No. k17-130, Shanghai, China). Informed consent was taken from all the patients.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.


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Cite this article as: Zhang W, Cao Z, Gao C, Huang Y, Wu C, Zhang L, Hou L. High concordance of programmed death-ligand 1 expression with immunohistochemistry detection between antibody clones 22C3 and E1L3N in non-small cell lung cancer biopsy samples. Transl Cancer Res 2020;9(10):5819-5828. doi: 10.21037/tcr-20-101