TY - JOUR AU - Zhou, Yan AU - Wen, Ping AU - Yu, Xian- Jing AU - Zuo, Luo AU - Wang, Ruixue AU - Liao, Xing AU - Li, La- Mei AU - Gao, Long AU - Li, Xiao- An PY - 2017 TI - Magnolol inhibits tumor cell growth in human pancreatic cancer cell JF - Translational Cancer Research; Vol 6, No 5 (October 31, 2017): Translational Cancer Research Y2 - 2017 KW - N2 - Background: Pancreatic cancer is the seventh most lethal malignancy owing to its rapid progression to advanced stages and resistance to conventional treatments. Magnolol is a natural compound with demonstrated anticancer properties. The effects and mechanism of action of magnolol on human pancreatic cancer are not known. To address this issue, we investigated the anti-tumor effects of magnolol in four human pancreatic cancer cell lines. Methods: PANC-1, CFPAC-1, ASPC-1, and MIAPaCa-2 cells were treated with magnolol (20–120 μM). Cell proliferation was evaluated with Cell Counting Kit-8 (CCK-8). Cell apoptosis was detected by flow cytometry. Cell migration was assessed with the transwell assay. Cathepsin D (CTSD) expression was evaluated by quantitative real-time PCR and western blotting, and its role in relation to the effects of magnolol was evaluated by RNA interference. Results: We found that magnolol inhibited proliferation and migration in all four cell lines while inducing apoptosis. Furthermore, the inhibition of migration by magnolol was mediated via inhibition of CTSD expression. Conclusions: Magnolol inhibits pancreatic cancer growth, and can thus serve as a natural alternative to chemotherapy. Keywords: Pancreatic cancer; magnolol; cathepsin D (CTSD); migration UR - https://tcr.amegroups.org/article/view/15809