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miR-1290 inhibits chordoma cell proliferation and invasion by targeting Robo1

  
@article{TCR27792,
	author = {Bin Wang and Kai Zhang and Hao Chen and Jian Lu and Guizhong Wu and Huilin Yang and Kangwu Chen},
	title = {miR-1290 inhibits chordoma cell proliferation and invasion by targeting Robo1},
	journal = {Translational Cancer Research},
	volume = {8},
	number = {2},
	year = {2019},
	keywords = {},
	abstract = {Background: Chordoma is a low-grade aggressive bone tumor with a high local recurrence. MicroRNAs (miRNAs) have been reported to play crucial roles in the development of chordoma. Our previous study has shown miR-1290 is associated with muscle invasion and the prognosis of chordoma. However, the underlying mechanism of miR-1290 in chordoma remains unclear. In this study, we aimed to explore the function of miR-1290 in the biological behaviors of chordoma. 
Methods: Sixteen sacral chordoma samples and 10 fetal nucleus pulposus specimens were collected for the detection of miR-1290 and Robo1 at the First Affiliated Hospital of Soochow University. Bioinformatic analysis and a luciferase reporter assay was used to verify the interaction between miR-1290 and the target gene robo1 in chordoma. Effects of miR-1290 expression on chordoma cell proliferation and invasion were explored by clone formation and Transwell assay in vitro. The underlying mechanisms of miR-1290 and Robo1 in chordoma cell proliferation and invasion were also explored in the U-CH1 cell line.
Results: In vitro functional analysis, including clone formation, and Transwell assays indicated overexpression of miR-1290 significantly suppressed chordoma cell proliferation and invasion. Bioinformatic analysis revealed Robo1 as a potential target of miR-1290, and luciferase reporter assays demonstrated the association between miR-1290 and the Robo1 gene in U-CH1 cells. Robo1 was further confirmed to be up-regulated in chordoma tissues by immunohistochemistry (IHC), which is negatively correlated with miR-1290 expression in chordoma tissue. Additionally, we found down-regulation of miR-1290 could induce the expression of Robo1 in chordoma cells, while the elevation of miR-1290 expression could inhibit Robo1 expression in chordoma cells. 
Conclusions: miR-1290 inhibits chordoma cell proliferation and invasion by negatively regulating the Robo1 gene.},
	issn = {2219-6803},	url = {http://tcr.amegroups.com/article/view/27792}
}