@article{TCR30695,
author = {Hua Jiang and Ting Zhang and Mao-Xiao Yan and Wei Wu},
title = {IL-35 inhibits CD8 + T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production},
journal = {Translational Cancer Research},
volume = {8},
number = {4},
year = {2019},
keywords = {},
abstract = {Background: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte.
Methods: Dendritic cells (DCs) were used to induce anti-tumor cytotoxic lymphocyte. Flow cytometry, carboxyfluorescein succinimidyl ester staining, ELISA assay and western blotting were used to analyze the effect of IL-35 on anti-tumor cytotoxic lymphocyte.
Results: We observed that IL-35 inhibited the expression of costimulatory molecule CD28 on CD8+ T cell surface and Th1 cytokine production. However, IL-35 did not inhibit anti-tumor cytotoxic lymphocyte proliferation nor enhance the expression of apoptosis-related proteins of anti-tumor cytotoxic lymphocyte. Moreover, IL-35 did not repress the expression of Fas ligand (FasL) on cytotoxic lymphocyte surface.
Conclusions: Our findings revealed that IL-35 can inhibit CD8+ T cells activity by suppressing the expression of costimulatory molecule CD28 and Th1 cytokine production.},
issn = {2219-6803}, url = {https://tcr.amegroups.org/article/view/30695}
}