TY - JOUR AU - Jiang, Hua AU - Zhang, Ting AU - Yan, Mao-Xiao AU - Wu, Wei PY - 2019 TI - IL-35 inhibits CD8 + T cells activity by suppressing expression of costimulatory molecule CD28 and Th1 cytokine production JF - Translational Cancer Research; Vol 8, No 4 (August 31, 2019): Translational Cancer Research Y2 - 2019 KW - N2 - Background: Interleukin-35 (IL-35), a novel immune-suppressing cytokine, can promote tumor angiogenesis and inhibits anti-tumor cytotoxic lymphocyte response. Here, we aimed to investigate the potential mechanism of the effects of IL-35 on anti-tumor cytotoxic lymphocyte. Methods: Dendritic cells (DCs) were used to induce anti-tumor cytotoxic lymphocyte. Flow cytometry, carboxyfluorescein succinimidyl ester staining, ELISA assay and western blotting were used to analyze the effect of IL-35 on anti-tumor cytotoxic lymphocyte. Results: We observed that IL-35 inhibited the expression of costimulatory molecule CD28 on CD8 + T cell surface and Th1 cytokine production. However, IL-35 did not inhibit anti-tumor cytotoxic lymphocyte proliferation nor enhance the expression of apoptosis-related proteins of anti-tumor cytotoxic lymphocyte. Moreover, IL-35 did not repress the expression of Fas ligand (FasL) on cytotoxic lymphocyte surface. Conclusions: Our findings revealed that IL-35 can inhibit CD8 + T cells activity by suppressing the expression of costimulatory molecule CD28 and Th1 cytokine production. UR - https://tcr.amegroups.org/article/view/30695