TY - JOUR AU - Chen, Fuhai AU - Zheng, Anyuan AU - Li, Fen AU - Wen, Silu AU - Chen, Shiming AU - Tao, Zezhang PY - 2019 TI - In vivo and in vitro investigation of KIN-193 anti-tumor effects on nasopharyngeal carcinoma JF - Translational Cancer Research; Vol 9, No 1 (January 18, 2020): Translational Cancer Research Y2 - 2019 KW - N2 - Background: The PI3K signaling pathway has important roles in nasopharyngeal carcinoma (NPC) tumorigenesis and progression. Inhibition of the PI3K pathway effectively inhibits NPC growth; however, the toxic side effects of PI3K inhibitors limit their clinical application. This study aimed to investigate the effects of the selective PI3K p110β inhibitor, KIN-193, on proliferation and apoptosis in NPC. Methods: Cell counting Kit-8, colony formation, flow cytometry, and western blotting experiments were conducted in CNE2Z NPC cells treated with various concentrations of KIN-193 to determine its effects on cell proliferation and apoptosis. Additionally, xenograft tumor models were established in nude mice and the anti-tumor effects of KIN-193 and the classical P110α inhibitor, PIK-75, compared in vivo . Hematoxylin- eosin (HE) staining, immunohistochemical staining, and western blotting were also conducted to detect the protein expression levels of proliferation and apoptosis markers. Results: The results of both in vivo and in vitro experiments demonstrated that KIN-193 can dramatically inhibit cell proliferation and promote apoptosis in NPC. In addition, KIN-193 showed stronger antitumor effects, with fewer side effects, than PIK-75 in vivo . Conclusions: We conclude that KIN-193 exhibits considerable anti-tumor effects in NPC. UR - https://tcr.amegroups.org/article/view/33710