%0 Journal Article %T Girdin knockdown promotes apoptosis in colorectal cancer via miR-29c-3p/Girdin axis %A Wang, Junjiang %A Li, Xiaowu %A Li, Yong %A Yao, Xueqing %J Translational Cancer Research %D 2019 %B 2019 %9 %! Girdin knockdown promotes apoptosis in colorectal cancer via miR-29c-3p/Girdin axis %K %X Background: In recent years, the incidence and mortality of colorectal cancer (CRC) have increased year by year among young people. Increased levels of Girdin expression predict a poor prognosis of CRC, which presents a serious threat to human health globally. Herein, we investigated the role of Girdin in CRC and explored the underlying mechanisms in CRC. Methods: The expression of Girdin was detected in human specimens. HCT116 cells with stably expressing or knock-out Girdin protein were successfully constructed to observe the biological function of gene. Protein expression was determined by immunohistochemistry, immunofluorescence and western blot. Results: Clinically, overexpression of Girdin was observed in the tumor tissue and poor prognosis of CRC patients. Gain-of-function and loss-of-function assays showed that Girdin promoted CRC cell proliferation in vitro . Mechanistically, Girdin knock-down significantly enhanced apoptosis, the mitochondrial membrane potential dropped, and the reactive oxygen species increased greatly. Last but not least, we analyzed the TargetScan dataset and found that Girdin was a regulated target of hsa-miR-29c-3p in CRC. Luciferase reporter assay was used to verify the interaction between hsa-miR-29c-3p and the 3’UTR of Girdin. Conclusions: Our findings suggest that Girdin has a crucial role in CRC progression via miR-29c-3p/ Girdin axis, highlighting Girdin as a therapeutic target for CRC. %U https://tcr.amegroups.org/article/view/34433 %V 8 %N 8 %P 2906-2915 %@ 2219-6803