TY - JOUR AU - Bao, Yejiang AU - Wang, Jia AU - Wang, Anjun AU - Bian, Jia AU - Jin, Yuan PY - 2020 TI - Correction of spurious low platelet counts by optical fluorescence platelet counting of BC-6800 hematology analyzer in EDTA-dependent pseudo thrombocytopenia patients JF - Translational Cancer Research; Vol 9, No 1 (January 18, 2020): Translational Cancer Research Y2 - 2020 KW - N2 - Background: To evaluate the dissociation effect of optical fluorescence platelet counting of BC-6800 hematology analyzer on ethylene diamine tetraacetic acid-dependent pseudo thrombocytopenia (EDTA- PTCP) samples. Methods: Twenty-three finally identified EDTA-PTCP samples were recruited in this study using criteria as follow: (I) impedance platelet counts lower than 100×10 9 /L with instrument “platelet aggregation” flag; (II) existence of platelet clumps in the blood smear; (III) obviously higher platelet counts without “platelet aggregation” flag and no platelet clumps in blood smear after repeating phlebotomy using citrate anticoagulated tubes. The BC-6800 hematology analyzer and the XE-2100 hematology analyzer were used to test 23 EDTA-PTCP samples and 30 controls on both the impedance channel and the reticulocyte channel. The dissociation rate was defined as optical fluorescence platelet counts in the EDTA tubes/impedance platelet counts in citrate tubes ×100%. Results: BC-6800 analyzer’s optical fluorescence platelet counts of EDTA-PTCP samples were significantly higher than impedance platelet counts (t=4.33, P=0.00) and comparable with the platelet counts of re-collected samples in tubes containing citrate anticoagulant. On BC-6800 hematology analyzer, 22 of 23 EDTA-PTCP samples showed a dissociation rate greater than 80%, and the average dissociation rate was 93%. On the XE-2100 hematology analyzer, 1 of the 17 EDTA-PTCP samples showed a dissociation rate greater than 80%, and the average dissociation rate was 56%. Conclusions: Optical fluorescence platelet counting of BC-6800 Hematology Analyzer is effective for the correction of spurious low platelet counts in EDTA-PTCP patients, and its dissociation effect on EDTA- PTCP samples was independent of fluorescent dye staining. UR - https://tcr.amegroups.org/article/view/34756