%0 Journal Article %T Potential roles of microRNA in levonorgestrel-treated uterine leiomyoma cells %A Ma, Huiyuan %A Shi, Xiaoyan %A Fu, Ziyi %A Wu, Xiaoli %A Huangfu, Yushuang %A Xu, Pengfei %A Zeng, Xin %A Xu, Juan %A Ruan, Hongjie %A Xu, Qing %J Translational Cancer Research %D 2018 %B 2018 %9 %! Potential roles of microRNA in levonorgestrel-treated uterine leiomyoma cells %K %X Background: Uterine leiomyoma is a common, benign, female tumor, which leads to gynecological disorders such as a recurrent pregnancy loss, and menstrual abnormalities. Although uterine leiomyoma can be cured by surgical and partial endocrine therapy, little is known about the biological mechanisms. Methods: In this study, uterine leiomyoma cells were treated with levonorgestrel (LNG) and prepared for microRNA (miRNA) microarray assay. The de-regulated miRNAs detected by microarray were confirmed by a quantitative polymerase chain reaction (qPCR). Gene ontology and pathway analysis were carried out to investigate the biological functions of these dys-regulated miRNAs. Results: The microarray data discovered 9 significantly up-regulated miRNAs and 32 predominantly down-regulated miRNAs in LNG-treated uterine leiomyoma cells. qPCR results further validated these discoveries. Gene ontology annotation showed that these dys-regulated miRNAs could regulate cell bicarbonate transport, phosphoinositide 3-kinase cascade, and other biological processes. Meanwhile, pathway analysis demonstrated that these dys-regulated miRNAs might participate in ErbB-MAPK signaling pathway regulation. Conclusions: The above results suggest that these dys-regulated miRNAs probably play a critical role in the progression of uterine leiomyoma. By providing new glimpses into the molecular mechanisms underlying the LNG intrauterine system (LNG-IUS) remedy, our study might be helpful in developing noninvasive clinical treatment for uterine leiomyoma. %U https://tcr.amegroups.org/article/view/25061 %V 7 %N 6 %P 1376-1383 %@ 2219-6803