Article Abstract

USP11 promotes tumorigenesis and enhances MRI detection in breast cancer

Authors: Ling Yang, Mo Zhu, Xinxing Ma, Chunhong Hu


Background: Ubiquitin-specific protease 11 (USP11) is a member of the deubiquitylating enzyme families. It usually works as an oncogene in some types of cancer such as colorectal and pancreatic cancers. But whether USP11 has a role in mediating tumorigenesis in breast cancer, the evidence is rare.
Methods: In order to evaluate the role of USP11 in breast cancer, qRT-PCR, western blot, cell growth assay, transwell assay and in vivo MRI assay was used. Co-immunoprecipitation assay and GST pull down assay revealed that the mechanism of USP11 contributes to the breast cancer progression in vivo and in vitro.
Results: The higher expression of USP11 was observed in the 120 breast cancer samples as well as the breast cancer cell lines compared with the normal breast tissues or normal breast mammary epithelial cell line. Furthermore, the higher expression of USP11 indicated a poorer overall 5-year survivals and worse prognosis. We identified USP11 played a critical role in the breast cancer cells proliferation, invasion and in vivo magnetic resonance imaging detection through counterbalanced Enhancer of zeste homologue 2 (EZH2) ubiquitination and stabilized it in MCF-7 cells and MDA-MB-231 cells.
Conclusions: Taken together, our study suggested that USP11 promoted breast cancer cell tumorigenesis and might work as a new biomarker and novel therapeutic target.